Advanced glycation end products (AGE) are formed during the Maillard reaction where reducing carbohydrates react with lysine side chains and N-terminal amino groups of various macromolecules, particularly proteins (Figure 1). The advanced glycation end products can adversely affect the fuction of these macromolecules. One of the most prevalent advanced glycation end products, N-epsilon-(Carboxymethyl) Lysine, has been implicated in oxidative stress and vascular damage. Our OxiSelect™ AGE Competitive ELISA test kit is designed for rapid detection and quantification of advanced glycation end product protein adducts. The quantity of AGE adduct in protein samples is determined by comparing its OD with that of a known AGE-BSA standard curve.
Human ELISAs or ELISA kits will be stored at +4°C. The expiry date is mostly determined by the standard stability. If your ELISA kit is expired we can supply a new standard so it will still be functional.
Upon receipt, aliquot and store the AGE-BSA Standard, AGE Conjugate and 100X Conjugate Diluent at -20ºC to avoid multiple freeze/thaw cycles. Store all other test kit components at 4ºC.
Oxidative Stress/ Damage, Protein Oxidation / Nitration, Advanced Glycation End Products, AGE Assays and Reagents, AGE Competitive ELISA
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,94
Detect advanced glycation end product formation as low as 0.5 µg/mL
Compatible with cell lysates, plasma, serum, and purified proteins
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
AGE-modified BSA and Reduced BSA included as standards
Enzyme-Linked Immunosorbent Assay kits
a specific protein sequence
in kilo Danlto or kDa