Cell Biolabs’ HIF-1 Cell Based ELISA test kit is an immunoassay developed for rapid detection of HIF-1 Alpha in fixed cells. Cells on a microplate are stimulated for HIF-1 Alpha stabilization, fixed, permeabilized, and then neutralized in the well. HIF-1 Alpha is then detected with an anti-HIF-1 alpha antibody followed by an HRP conjugated secondary antibody. Each test kit provides sufficient reagents to perform up to a total of 96 assays and can detect HIF-1 Alpha from human, mouse, or rat.
Human ELISAs or ELISA kits will be stored at +4°C. The expiry date is mostly determined by the standard stability. If your ELISA kit is expired we can supply a new standard so it will still be functional.
The HIF-1 Alpha Based ELISA test kit is a α- or alpha protein sometimes glycoprotein present in blood.For cells, cell lines and tissues in culture till half confluency.
Upon receipt, store the Anti-HIF-1 Alpha Antibody at -20ºC. Store all other components at 4ºC.
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,52
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Cell Based Assays, Cell Health Assays>Hypoxia Assays
human cells and cell lines and other cells
Enzyme-Linked Immunosorbent Assay kits
the sequence of the protein
Cell Based Assays