Size

96 assays

Price

954 EUR

Catalog no

VPK-156

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Full description

Below is a list of all the features of this product

Use of the reagent

An anti-MuLV p30 monoclonal coating antibody is adsorbed onto a microtiter plate. MuLV core antigen (p30) present in the sample or standard binds to the antibodies adsorbed on the plate; an anti-MuLV p30 polyclonal antibody is added and binds to the antigen captured by the first antibody. Following incubation and wash steps, a HRP-conjugated secondary antibody is added and binds to the anti-MuLV p30 polyclonal. Unbound HRP-conjugated secondary antibody is removed during the wash steps, and substrate solution reactive with HRP is added to the wells. A colored product is formed in proportion to the amount of MuLV core antigen present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from recombinant MuLV p30 core antigen and sample concentration is then determined.

Storage

Human ELISAs or ELISA kits will be stored at +4°C. The expiry date is mostly determined by the standard stability. If your ELISA kit is expired we can supply a new standard so it will still be functional.

The temperatureerature for storage is

Upon receipt, aliquot and store the Recombinant MuLV p30 Standard at -20ºC to avoid multiple freeze/thaw cycles. Store all other test kit components at 4ºC.

Technical file

kit.pdf

SDS or safty data sheet

(VPK-156) GHS.pdf

Related techniques

Pathogen and Toxin Assays, Virus Core Antigen Detection, MuLV Core Antigen ELISA test kit

Description

Antigens are peptides or recombinant or native dependent on the production method.

Buffer

The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,43

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Protocol information

quantification is performed on a standard microplate reader

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Cellular use

Measures the MuLV core protein (p30) as low as 300 pg/mL

Living modified organisme in this reagent or LMO

Recombinant Protein purified from E. coli

Group

Enzyme-Linked Immunosorbent Assay kits

Reagent properties

Recombinant MuLV p30 Standard included

Antigen

a specific protein sequence

Type of reagent

Pathogen and Toxin Assays

Molecular weight

in kilo Danlto or kDa

Use of this test kit or product

Genetics lab

We send this reagant on

Blue Ice

Species compatible to

Humans

This reagent is purified

0.97

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