An anti-MuLV p30 monoclonal coating antibody is adsorbed onto a microtiter plate. MuLV core antigen (p30) present in the sample or standard binds to the antibodies adsorbed on the plate; an anti-MuLV p30 polyclonal antibody is added and binds to the antigen captured by the first antibody. Following incubation and wash steps, a HRP-conjugated secondary antibody is added and binds to the anti-MuLV p30 polyclonal. Unbound HRP-conjugated secondary antibody is removed during the wash steps, and substrate solution reactive with HRP is added to the wells. A colored product is formed in proportion to the amount of MuLV core antigen present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from recombinant MuLV p30 core antigen and sample concentration is then determined.
Human ELISAs or ELISA kits will be stored at +4°C. The expiry date is mostly determined by the standard stability. If your ELISA kit is expired we can supply a new standard so it will still be functional.
Upon receipt, aliquot and store the Recombinant MuLV p30 Standard at -20ºC to avoid multiple freeze/thaw cycles. Store all other test kit components at 4ºC.
Pathogen and Toxin Assays, Virus Core Antigen Detection, MuLV Core Antigen ELISA test kit
Antigens are peptides or recombinant or native dependent on the production method.
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,43
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
quantification is performed on a standard microplate reader
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Measures the MuLV core protein (p30) as low as 300 pg/mL
Recombinant Protein purified from E. coli
Enzyme-Linked Immunosorbent Assay kits
Recombinant MuLV p30 Standard included
a specific protein sequence
Pathogen and Toxin Assays
in kilo Danlto or kDa