The CytoSelect™ BrdU Cell Proliferation ELISA test kit detects BrdU incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody. When cells are incubated in media containing BrdU, the pyrimidine analog is incorporated in place of thymidine into the newly synthesized DNA of proliferating cells. Once the labeling media is removed, the cells are fixed and the DNA is denatured in one step with a fix/denature solution (denaturation of the DNA is necessary to improve the accessibility of the incorporated BrdU for detection). Then an anti-BrdU mouse monoclonal antibody is added followed by an HRP conjugated secondary antibody to detect the incorporated BrdU. The magnitude of the absorbance for the developed color is proportional to the quantity of BrdU incorporated into cells and can be directly correlated to cell proliferation.
Human ELISAs or ELISA kits will be stored at +4°C. The expiry date is mostly determined by the standard stability. If your ELISA kit is expired we can supply a new standard so it will still be functional.
Upon receipt, store the 1000X BrdU Solution and Anti-BrdU Monoclonal Antibody at -20ºC. Store all other components at 4ºC.
Detects BrdU incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody
Cell Based Assays, Cell Health Assays, Cell Proliferation Assays, BrdU Cell Proliferation ELISA
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,47
For cells, cell lines and tissues in culture till half confluency.
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
human cells and cell lines and other cells
Enzyme-Linked Immunosorbent Assay kits
the sequence of the protein
BrdU standard included
Cell Based Assays