Advanced glycation end products (AGE) are formed during the Maillard reaction where reducing carbohydrates react with lysine side chains and N-terminal amino groups of various macromolecules, particularly proteins. The advanced glycation end products can adversely affect the fuction of these macromolecules. One of the most prevalent AGE products, N-epsilon-(Carboxymethyl) Lysine, has been implicated in oxidative stress and vascular damage. The OxiSelect™ N-epsilon-(Carboxymethyl) Lysine Competitive ELISA test kit specifically detects CML formation with a high level of sensitivity. This is a Competitive ELISA test kit in which the plate is coated with a CML conjugate. Standards and unknown samples are added to the plate, followed by incubation with the primary antibody. The CML in the unknown samples and the CML attached to the plate compete for the primary antibody. Higher CML content in unknown samples results in more binding of the antibody to the sample, and thus less antibody binds to the plate. Since the antibody bound to the sample is washed away, higher CML content in samples correlates with a lower signal.
Human ELISAs or ELISA kits will be stored at +4°C. The expiry date is mostly determined by the standard stability. If your ELISA kit is expired we can supply a new standard so it will still be functional.
Upon receipt, aliquot and store the CML-BSA Standard, CML Conjugate and 100X Conjugate Diluent at -20ºC to avoid multiple freeze/thaw cycles. Store all other test kit components at 4ºC.
Oxidative Stress/ Damage, Advanced Glycation End Products, CML Assays (N-epsilon-(Carboxymethyl) Lysine), CML Competitive ELISA
Compatible with cell lysates, serum, plasma, purified proteins, and other protein-containing samples
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,89
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Detect CML as low as 50 ng/mL from a variety of samples
Enzyme-Linked Immunosorbent Assay kits
CML-modified BSA included as standard
a specific protein sequence
in kilo Danlto or kDa