Our Cholic Acid ELISA test kit is designed for detection and quantification of cholic acid in plasma, serum, urine, feces, or lysates. This assay is a competitive ELISA where unknown samples are added to a plate pre-adsorbed with a cholic acid conjugate. An anti-cholic acid antibody is added, which is then detected by an HRP conjugated secondary antibody. Cholic acid levels in unknown samples are calculated based on a cholic acid standard curve.
Human ELISAs or ELISA kits will be stored at +4°C. The expiry date is mostly determined by the standard stability. If your ELISA kit is expired we can supply a new standard so it will still be functional.
Upon receipt, aliquot and store both the Anti-Cholic Acid Antibody and 100X Cholic Acid Conjugate at -20ºC and avoid multiple freeze/thaw cycles. Store all other components at 4ºC.
Metabolism Research, Lipoprotein Metabolism, Bile Acid Assays, Cholic Acid ELISA
Suitable for use with plasma, serum, urine, feces, and cell and tissue lysates
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,91
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Detection sensitivity limit of 0.4µM cholic acid
human cells and cell lines and other cells
Enzyme-Linked Immunosorbent Assay kits
Cholic acid standard included
the sequence of the protein