Size

5 x 96 assays

Price

3424 EUR

Catalog no

STA-816-5

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Full description

Below is a list of all the features of this product

Use of the reagent

Advanced glycation end products (AGE) are formed during the Maillard reaction where reducing carbohydrates react with lysine side chains and N-terminal amino groups of various macromolecules, particularly proteins. The advanced glycation end products can adversely affect the fuction of these macromolecules. One of the most prevalent AGE products, N-epsilon-(Carboxymethyl) Lysine, has been implicated in oxidative stress and vascular damage. The OxiSelect™ N-epsilon-(Carboxymethyl) Lysine Competitive ELISA test kit specifically detects CML formation with a high level of sensitivity. This is a Competitive ELISA test kit in which the plate is coated with a CML conjugate. Standards and unknown samples are added to the plate, followed by incubation with the primary antibody. The CML in the unknown samples and the CML attached to the plate compete for the primary antibody. Higher CML content in unknown samples results in more binding of the antibody to the sample, and thus less antibody binds to the plate. Since the antibody bound to the sample is washed away, higher CML content in samples correlates with a lower signal.

Storage

Human ELISAs or ELISA kits will be stored at +4°C. The expiry date is mostly determined by the standard stability. If your ELISA kit is expired we can supply a new standard so it will still be functional.

The temperatureerature for storage is

Upon receipt, aliquot and store the CML-BSA Standard, CML Conjugate and 100X Conjugate Diluent at -20ºC to avoid multiple freeze/thaw cycles. Store all other test kit components at 4ºC.

Related techniques

Oxidative Stress/ Damage, Advanced Glycation End Products, CML Assays (N-epsilon-(Carboxymethyl) Lysine), CML Competitive ELISA

SDS or safty data sheet

(STA-816) GHS.pdf

Reagent properties

Compatible with cell lysates, serum, plasma, purified proteins, and other protein-containing samples

Buffer

The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,90

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Cellular use

Detect CML as low as 50 ng/mL from a variety of samples

Group

Enzyme-Linked Immunosorbent Assay kits

Protocol information

CML-modified BSA included as standard

Antigen

a specific protein sequence

Type of reagent

Oxidative Stress/Damage

Molecular weight

in kilo Danlto or kDa

Living modified organisme in this reagent or LMO

Not applicable

Use of this test kit or product

Genetics lab

We send this reagant on

Blue Ice

Species compatible to

Humans

This reagent is purified

0.97

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