Nitric oxide influences a variety of biological processes including cell proliferation, apoptosis, neurotoxicity and extracellular matrix remodeling. Nitric oxide reacts with superoxide to form peroxynitrite, which in turn nitrates tyrosine residues in proteins. Nitrotyrosine therefore serves as a marker for peroxynitrite action in a variety of disease states and in conditions of cellular damage and oxidative stress. Our OxiSelect™ Nitrotyrosine ELISA test kit provides a sensitive method to measure the formation of 3-nitrotyrosine in proteins.
Human ELISAs or ELISA kits will be stored at +4°C. The expiry date is mostly determined by the standard stability. If your ELISA kit is expired we can supply a new standard so it will still be functional.
Upon receipt, aliquot and store the Nitrated BSA Standard at -20ºC to avoid multiple freeze/thaw cycles. Store all other test kit components at 4ºC.
Oxidative Stress/ Damage, Protein Oxidation / Nitration, Protein Nitration Assays and Reagents
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,29
Suitable for use with cell lysates, serum, plasma and purified proteins
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Sensitive detection of 3-Nitrotyrosine as low as 10 nM
Nitrated BSA provided as positive control
Enzyme-Linked Immunosorbent Assay kits
a specific protein sequence
in kilo Danlto or kDa