S-Adenosylmethionine (SAM) ELISA test kit

96 assays

677 EUR

STA-672

S-adenosylmethionine (SAM) is a substrate for methyl transfers to various biomolecules that yields S-adenosylhomocysteine (SAH). SAH is then hydrolyzed to homocysteine and adenosine, which is converted to methionine, and ultimately back to SAM. SAH can act as a methylation inhibitor, therefore the SAM/SAH ratio, or methylation potential, is important for maintaining proper methylation in the cell. Our S-adenosylmethionine (SAM) ELISA is designed for detection and quantification of SAM in plasma, serum, lysates, or biological fluid samples. This assay is a competitive ELISA where samples and an anti-SAM antibody are added to a plate coated with a SAM conjugate. The sample and conjugate compete for antibody binding, which generates a reverse curve. Samples with high SAM levels will bind the majority of the antibody, which gets washed away and results in a low OD, while samples with low SAM levels will leave more antibody available to bind to the conjugate, producing a high signal.

Human ELISAs or ELISA kits will be stored at +4°C. The expiry date is mostly determined by the standard stability. If your ELISA kit is expired we can supply a new standard so it will still be functional.

kit.pdf

(STA-672) GHS.pdf

Upon receipt, store SAM Conjugate and SAM-BSA Standard at -80ºC. Store the rest of the test kit at 4ºC.

Metabolism Research, Amino Acid Metabolism, S-Adenosylmethionine (SAM) ELISA test kit

Suitable for use with plasma, serum, lysates, and other biological fluid samples

The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,58

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Detection sensitivity limit of 185ng/mL SAM-BSA

Enzyme-Linked Immunosorbent Assay kits

a specific protein sequence

in kilo Danlto or kDa

Metabolism Research

Genetics lab

Blue Ice

Humans

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