The OxiSelect™ Aldehyde-Induced DNA Damage ELISA test kit (Ethenocytidine quantification) is a competitive ELISA test kit allowing the quantification of ethenocytidine adducts in DNA isolated from cells or tissues. Samples and standards are added to a provided microplate preabsorbed with etheno-damaged DNA. An anti-ethenocytidine antibody is added and binds competitively to ethenocytidine in the unknown sample or to the ethenocytidine in the DNA coated on the plate. After washing a secondary antibody is added. A low signal means most of the primary antibody was bound to the unknown, indicating a high level of ethenocytidine in the sample.
Human ELISAs or ELISA kits will be stored at +4°C. The expiry date is mostly determined by the standard stability. If your ELISA kit is expired we can supply a new standard so it will still be functional.
Upon receipt, store the Ethenocytidine Standard and Anti-Ethenocytidine Antibody at -20ºC. Store all other components at 4ºC until their expiration dates.
Induced protein genes, factors or kinases, increase the production of (an enzyme or other protein) at the level of genetic transcription.
Oxidative Stress/ Damage, DNA / RNA Damage and Repair, Aldehyde Induced DNA Damage Assays, Ethenocytidine ELISA
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,01
Unknown samples are compared with a provided ethenocytidine standard
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Suitable for use with DNA isolated from cells or tissues
Detect as little as 625 nM of ethenocytidine
Enzyme-Linked Immunosorbent Assay kits
a specific protein sequence
in kilo Danlto or kDa