Homocysteine is an amino acid intermediate that is synthesized from methionine via S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH). Elevated levels of homocysteine can indicate a risk for numerous diseases, including vascular and heart disease. Our Homocysteine ELISA is designed for detection and quantification of homocysteine in plasma, serum, lysates, or biological fluid samples. This assay is a competitive ELISA where samples and an anti-homocysteine antibody are added to a plate coated with a homocysteine conjugate. The sample and conjugate compete for antibody binding, which generates a reverse curve. Samples with high homocysteine levels will bind to most of the antibody, which gets washed away and results in a low OD, while samples with low homocysteine levels will leave more antibody available to bind to the conjugate, producing a high signal.
Human ELISAs or ELISA kits will be stored at +4°C. The expiry date is mostly determined by the standard stability. If your ELISA kit is expired we can supply a new standard so it will still be functional.
Upon receipt, store Homocysteine Conjugate (1000X) and Homocysteine-BSA Standard at -20ºC. Store the rest of the test kit at 4ºC.
Suitable for use with plasma, serum, lysates, and other biological fluid samples
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,55
Metabolism Research, Amino Acid Metabolism, Homocysteine ELISA test kit
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Detection sensitivity limit of 10ng/mL Homocysteine-BSA
Enzyme-Linked Immunosorbent Assay kits
a specific protein sequence
in kilo Danlto or kDa