The OxiSelect™ Aldehyde-Induced DNA Damage ELISA test kit (Ethenoadenosine quantification) is a competitive ELISA test kit allowing the quantification of ethenoadenosine adducts in DNA isolated from cells or tissues. Samples and standards are added to a provided microplate preabsorbed with etheno-damaged DNA. An anti-ethenoadenosine antibody is added and binds competitively to ethenoadenosine in the unknown sample or to the ethenoadenosine in the DNA coated on the plate. After washing a secondary antibody is added. A low signal means most of the primary antibody was bound to the unknown, indicating a high level of ethenoadenosine in the sample.
Human ELISAs or ELISA kits will be stored at +4°C. The expiry date is mostly determined by the standard stability. If your ELISA kit is expired we can supply a new standard so it will still be functional.
Upon receipt, store the Ethenoadenosine Standard and Anti-Ethenoadenosine Antibody at -20ºC. Store all other components at 4ºC until their expiration dates.
Induced protein genes, factors or kinases, increase the production of (an enzyme or other protein) at the level of genetic transcription.
Oxidative Stress/ Damage, DNA / RNA Damage and Repair, Aldehyde Induced DNA Damage Assays, Ethenoadenosine ELISA
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,95
Unknown samples are compared with a provided ethenoadenosine standard
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Suitable for use with DNA isolated from cells or tissues
Detect as little as 50 nM of ethenoadenosine
Enzyme-Linked Immunosorbent Assay kits
a specific protein sequence
in kilo Danlto or kDa