BSA standards or protein samples (10 μg/mL) are adsorbed onto a 96-well plate for 2 hrs at 37ºC. The BPDE-protein adducts present in the sample or standard are probed with an Anti-BPDE-I Antibody, followed by an HRP Conjugated Secondary Antibody. The BPDE protein adduct content in an unknown sample is determined by comparing with a standard curve that is prepared from predetermined BPDE-BSA standards.
Human ELISAs or ELISA kits will be stored at +4°C. The expiry date is mostly determined by the standard stability. If your ELISA kit is expired we can supply a new standard so it will still be functional.
Upon receipt, aliquot and store the Reduced BSA and BPDE-BSA Standards at -20ºC to avoid multiple freeze/thaw cycles. Store all other test kit components at 4ºC.
Suitable for use with protein samples such as purified protein, plasma, serum, or cell lysates
For detection and quantification of benzo(a)pyrene diol epoxide (BPDE) protein adducts
Oxidative Stress/ Damage, Protein Oxidation / Nitration, BPDE Protein Adduct ELISA
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,23
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Enzyme-Linked Immunosorbent Assay kits
a specific protein sequence
BPDE-BSA standard included
in kilo Danlto or kDa