First, a CEL conjugate is coated on the ELISA plate. The unknown CEL protein samples or CEL-BSA standards are then added to the CEL conjugate preabsorbed plate. After a brief incubation, the anti-CEL monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The content of CEL protein adducts in unknown samples is determined by comparison with the predetermined CEL-BSA standard curve. Despite the structure similarity between CEL and CML, the anti-CEL specific antibody in the OxiSelect™ CEL ELISA test kit will not cross react with CML protein adducts.
Human ELISAs or ELISA kits will be stored at +4°C. The expiry date is mostly determined by the standard stability. If your ELISA kit is expired we can supply a new standard so it will still be functional.
Upon receipt, aliquot and store the CEL-BSA Standard, CEL Conjugate and 100X Conjugate Diluent at -20ºC to avoid multiple freeze/thaw cycles. Store all other test kit components at 4ºC.
Oxidative Stress/ Damage, Protein Oxidation / Nitration, Advanced Glycation End Products, CEL (N-epsilon-(Carboxyethyl) Lysine) Assays and Reagents, CEL Competitive ELISA
Perform up to 96 assays, including standard curve and unknown protein samples
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,87
Provides rapid detection and quantification of CEL protein adducts
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Will not cross react with CML protein adducts
Enzyme-Linked Immunosorbent Assay kits
a specific protein sequence
in kilo Danlto or kDa