Glutathione helps protect cells from free radical damage as an antioxidant, but it can also covalently attach to proteins through its sulfhydryl moiety upon derivatization by reactive oxygen species (ROS). Glutathione protein adducts can therefore serve as a marker of oxidative stress. OxiSelect s-Glutathione Adduct Competitive ELISA test kit quantifies the glutathionylation of proteins in a standard 96-well plate.
Upon receipt, aliquot and store the Anti-s-Glutathione Adduct Antibody, s-Glutathione-BSA Standard, s-Glutathione Conjugate and 100X Conjugate Diluent at -20ºC to avoid multiple freeze/thaw cycles. Store all other components at 4ºC.
Human ELISAs or ELISA kits will be stored at +4°C. The expiry date is mostly determined by the standard stability. If your ELISA kit is expired we can supply a new standard so it will still be functional.
Oxidative Stress/ Damage, Protein Oxidation / Nitration, Protein Glutathionylation Adduct ELISA
Suitable for use with protein samples from cells, lysates, plasma or serum
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,88
Quantify s-Glutathione protein adducts in a competitive ELISA format
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Read results in a standard 96-well colorimetry plate reader
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Enzyme-Linked Immunosorbent Assay kits
a specific protein sequence
in kilo Danlto or kDa