An anti-HCVcAg monoclonal coating antibody is adsorbed onto a microtiter plate. HCV core antigen present in the sample or standard binds to the antibodies adsorbed on the plate; a FITC-conjugated mouse anti-HCVcAg antibody is added and binds to the antigen captured by the first antibody. Following incubation and wash steps, a HRP-conjugated mouse anti-FITC antibody is added and binds to the FITC conjugated anti-HCVcAg. Following unbound HRP-conjugated mouse anti-FITC antibody is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A colored product is formed in proportion to the amount of HCV core antigen present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from recombinant HCV core antigen and sample concentration is then determined.
Hepatitis C virus (HCV) NS3, NS4, NS genotypes 1, 2, 3, 4, 5, are small (55–65 nm in size), enveloped, positive-sense single-stranded RNA viruses. Hepatitis C virus is the cause of hepatitis C and some cancers such as Liver Cancer (Hepatocellular carcinoma abbreviated HCC) and lymphomas in humans. HCV Core antigens or envelope proteins are used for immunization to obtain diagnostic antibodies.
Human ELISAs or ELISA kits will be stored at +4°C. The expiry date is mostly determined by the standard stability. If your ELISA kit is expired we can supply a new standard so it will still be functional.
Upon receiving, aliquot and store recombinant HCVcAg Standard at -20ºC and avoid freeze/thaw. Store all other components at 4ºC.
Antigens are peptides or recombinant or native dependent on the production method.
Pathogen and Toxin Assays, Virus Core Antigen Detection, HCV Core Antigen ELISA
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,42
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Measures the core antigen of Hepatitis C virus
Recombinant Protein purified from E. coli
Enzyme-Linked Immunosorbent Assay kits
Recombinant HCVcAg standard included
Sensitive to as low as 1 ng/mL
a specific protein sequence
Pathogen and Toxin Assays
in kilo Danlto or kDa