S-adenosylhomocysteine (SAH) is generated through the donation of a methyl group from S-adenosylmethionine (SAM) to various biomolecules. SAH is then hydrolyzed to homocysteine and adenosine, which is converted to methionine, and ultimately back to SAM. SAH can act as a methylation inhibitor, therefore the SAM/SAH ratio, or methylation potential, is important for maintaining proper methylation in the cell. Our S-adenosylhomocysteine (SAH) ELISA is designed for detection and quantification of SAH in plasma, serum, lysates, or biological fluid samples. This assay is a competitive ELISA where samples and an anti-SAH antibody are added to a plate coated with a SAH conjugate. The sample and conjugate compete for antibody binding, which generates a reverse curve. Samples with high SAH levels will bind the majority of the antibody, which gets washed away and results in a low OD, while samples with low SAH levels will leave more antibody available to bind to the conjugate, producing a high signal.
Human ELISAs or ELISA kits will be stored at +4°C. The expiry date is mostly determined by the standard stability. If your ELISA kit is expired we can supply a new standard so it will still be functional.
Upon receipt, store SAH Conjugate and SAH-BSA Standard at -80ºC. Store the rest of the test kit at 4ºC.
Metabolism Research, Amino Acid Metabolism, S-Adenosylhomocysteine (SAH) ELISA test kit
Suitable for use with plasma, serum, lysates, and other biological fluid samples
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,56
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Detection sensitivity limit of 7ng/mL SAH-BSA
Enzyme-Linked Immunosorbent Assay kits
a specific protein sequence
in kilo Danlto or kDa