Size

96 assays

Price

954 EUR

Catalog no

STA-441

Simple buy process

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Full description

Below is a list of all the features of this product

Use of the reagent

Our 96-Well Ras Activation ELISA test kit uses the Raf1 RBD (Rho binding domain) bound to a 96-well plate to selectively pull down the active form of Ras from purified or endogenous samples. The captured GTP-Ras is then detected by a pan-Ras antibody and HRP-conjugated secondary antibody.

The temperatureerature for storage is

Upon receipt, aliquot and store Raf-1 RBD at -80ºC and avoid freeze/thaw. Aliquot and store the Anti-pan-Ras Antibody, Secondary Antibody, GTPγS, and GDP components at -20ºC and avoid freeze/thaw. Store all other components at 4ºC until their expiration dates.

Storage

Human ELISAs or ELISA kits will be stored at +4°C. The expiry date is mostly determined by the standard stability. If your ELISA kit is expired we can supply a new standard so it will still be functional.

Related techniques

Cell Signaling and Protein Biology, G Protein Signaling / Small GTPase Assays and Reagents, Small GTPase Research Tools, Small GTPase Activation ELISA test kits, Ras Activation ELISA, Chemiluminescent

SDS or safty data sheet

(STA-441) GHS.pdf

Buffer

The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,12

Protocol information

Convenient 96-well format for use with any plate-based luminometer

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Cellular use

Raf1 RBD detects only the active form of Ras

Group

Enzyme-Linked Immunosorbent Assay kits

Type of reagent

Cell Signaling and Protein Biology

Antigen

a specific protein sequence

Molecular weight

in kilo Danlto or kDa

Use of this test kit or product

Genetics lab

We send this reagant on

Blue Ice

Species compatible to

Humans

This reagent is purified

0.97

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