4-hydroxynonenal (4-HNE or HNE) is a well known by-product of lipid peroxidation and is widely accepted as a stable marker for oxidative stress. Our OxiSelect™ HNE Adduct Competitive ELISA test kit measures the formation of HNE adducts to any protein residue using a competitive ELISA format.
Human ELISAs or ELISA kits will be stored at +4°C. The expiry date is mostly determined by the standard stability. If your ELISA kit is expired we can supply a new standard so it will still be functional.
Upon receipt, aliquot and store the HNE-BSA Standard, HNE Conjugate and 100X Conjugate Diluent at -20ºC to avoid multiple freeze/thaw cycles. Store all other test kit components at 4ºC.
Measure HNE protein adduct levels in a variety of samples including cell and tissue lysates, serum, plasma, and purified proteins
Oxidative Stress/ Damage, Lipid Peroxidation, HNE (4-Hydroxynonenal) Assays and Reagents, HNE Adduct Competitive ELISA
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,07
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Enzyme-Linked Immunosorbent Assay kits
a specific protein sequence
HNE-BSA standard included
in kilo Danlto or kDa